LZTR1 Knockout cell line (Hep 3B2.1-7)

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LZTR1 Knockout cell line (Hep 3B2.1-7) Order

LZTR1 Knockout cell line (Hep 3B2.1-7)
Catalog#
YKO-H844
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LZTR1 Knockout cell line (Hep 3B2.1-7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LZTR1 Knockout cell line（Hep 3B）
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
LZTR1
Gene ID
8216
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
87%MEM+10% FBS+1%Glutamax+1%Sodium Pyruvate+1%NEAA
Accession
CVCL_D9W5(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 1 (based on transcript LZTR1-221)
Validation: PCR+Sanger Sequencing
LZTR1 Knockout cell line (Hep 3B2.1-7) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:Hep 3B		 STR Info (Cell bank)
Cell Line:HEP-3B
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 12141214
D7S820 810810
D16S539 10101010
VWA 17171717
TH01 6767
AMEL XXXX
TPOX 9999
CSF1PO 8888
D12S391 1717
FGA 1818
D2S1338 2125
D21S11 3031
D18S51 2020
D8S1179 1212
D3S1358 1515
D6S1043 1217
PENTAE 516
D19S433 12.214
PENTAD 1214
D1S1656 1717.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LZTR1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep 3B cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LZTR1 gene knockout LZTR1 Knockout cell line（Hep 3B） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LZTR1 Knockout cell line (Hep 3B2.1-7)
LZTR1 Knockout cell line (Hep 3B2.1-7)
Catalog#
YKO-H844
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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